FREEZING OF BUCK SEMEN WITH DIFFERENT GLYCEROL CONCENTRATIONS

Abstract

Effect of different concentrations of glycerol on post-thaw motility and fertility of frozen buck semen was studied using semen from five Malabari crossbred bucks. Average percentage sperm motility after washing in Tris buffer was 76.71 ± 0.79. Maxirnutn percentage motility after glycerolisation (67.8.5 ± 1.39) and freezing (42.0 ± 1.84) was obtained in six per cent glycerolated semen. There was highly ignificant reduction in motility percentage after glycerolisation and freezing. Conception percentage and kidding percentage were 47.36 and 43.85 respectively with 6% glycerolated samples.