An Igh distal enhancer modulates antigen receptor diversity by determining locus conformation

Khalid H. Bhat; Saurabh Priyadarshi; Sarah Naiyer; Xinyan Qu; Hammad Farooq; Eden Kleiman; Jeffery Xu; Xue Lei; Jose F. Cantillo; Robert Wuerffel; Nicole Baumgarth; Jie Liang; Ann J. Feeney; Amy L. Kenter

doi:10.1038/s41467-023-36414-2

Nature Communications (Mar 2023)

An Igh distal enhancer modulates antigen receptor diversity by determining locus conformation

Khalid H. Bhat,
Saurabh Priyadarshi,
Sarah Naiyer,
Xinyan Qu,
Hammad Farooq,
Eden Kleiman,
Jeffery Xu,
Xue Lei,
Jose F. Cantillo,
Robert Wuerffel,
Nicole Baumgarth,
Jie Liang,
Ann J. Feeney,
Amy L. Kenter

Affiliations

Khalid H. Bhat: Department of Microbiology and Immunology, University of Illinois College of Medicine
Saurabh Priyadarshi: Department of Microbiology and Immunology, University of Illinois College of Medicine
Sarah Naiyer: Department of Microbiology and Immunology, University of Illinois College of Medicine
Xinyan Qu: Department of Microbiology and Immunology, University of Illinois College of Medicine
Hammad Farooq: Department of Bioengineering, University of Illinois Colleges of Engineering and Medicine
Eden Kleiman: Department of Immunology and Microbiology, IMM-22, Scripps Research
Jeffery Xu: Department of Immunology and Microbiology, IMM-22, Scripps Research
Xue Lei: Department of Bioengineering, University of Illinois Colleges of Engineering and Medicine
Jose F. Cantillo: Department of Microbiology and Immunology, University of Illinois College of Medicine
Robert Wuerffel: Department of Microbiology and Immunology, University of Illinois College of Medicine
Nicole Baumgarth: W. Harry Feinstone Dept. Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University
Jie Liang: Department of Bioengineering, University of Illinois Colleges of Engineering and Medicine
Ann J. Feeney: Department of Immunology and Microbiology, IMM-22, Scripps Research
Amy L. Kenter: Department of Microbiology and Immunology, University of Illinois College of Medicine

DOI: https://doi.org/10.1038/s41467-023-36414-2
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 18

Abstract

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Here the authors show enhancers in the Igh locus play a major role in configuring locus architecture and in V(D)J recombination, and identify a link between enhancer hub formation, locus contraction and cohesin-mediated loop extrusion.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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