Nature Communications (Mar 2023)
Limitations of gene editing assessments in human preimplantation embryos
- Dan Liang,
- Aleksei Mikhalchenko,
- Hong Ma,
- Nuria Marti Gutierrez,
- Tailai Chen,
- Yeonmi Lee,
- Sang-Wook Park,
- Rebecca Tippner-Hedges,
- Amy Koski,
- Hayley Darby,
- Ying Li,
- Crystal Van Dyken,
- Han Zhao,
- Keliang Wu,
- Jingye Zhang,
- Zhenzhen Hou,
- Seongjun So,
- Jongsuk Han,
- Jumi Park,
- Chong-Jai Kim,
- Kai Zong,
- Jianhui Gong,
- Yilin Yuan,
- Ying Gu,
- Yue Shen,
- Susan B. Olson,
- Hui Yang,
- David Battaglia,
- Thomas O’Leary,
- Sacha A. Krieg,
- David M. Lee,
- Diana H. Wu,
- P. Barton Duell,
- Sanjiv Kaul,
- Jin-Soo Kim,
- Stephen B. Heitner,
- Eunju Kang,
- Zi-Jiang Chen,
- Paula Amato,
- Shoukhrat Mitalipov
Affiliations
- Dan Liang
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Aleksei Mikhalchenko
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Hong Ma
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Nuria Marti Gutierrez
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Tailai Chen
- Center for Reproductive Medicine, Shandong University
- Yeonmi Lee
- Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University
- Sang-Wook Park
- Center for Genome Engineering, Institute for Basic Science, 70, Yuseong-daero 1689-gil, Yuseong-gu
- Rebecca Tippner-Hedges
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Amy Koski
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Hayley Darby
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Ying Li
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Crystal Van Dyken
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Han Zhao
- Center for Reproductive Medicine, Shandong University
- Keliang Wu
- Center for Reproductive Medicine, Shandong University
- Jingye Zhang
- Center for Reproductive Medicine, Shandong University
- Zhenzhen Hou
- Center for Reproductive Medicine, Shandong University
- Seongjun So
- Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University
- Jongsuk Han
- Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University
- Jumi Park
- Stem Cell Center, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu
- Chong-Jai Kim
- Stem Cell Center, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu
- Kai Zong
- Technology Center of Hefei Customs
- Jianhui Gong
- Guangdong Provincial Key Laboratory of Genome Read and Write
- Yilin Yuan
- Guangdong Provincial Key Laboratory of Genome Read and Write
- Ying Gu
- Guangdong Provincial Key Laboratory of Genome Read and Write
- Yue Shen
- Guangdong Provincial Key Laboratory of Genome Read and Write
- Susan B. Olson
- Department of Molecular and Medical Genetics and Knight Diagnostic Laboratories, Oregon Health & Science University
- Hui Yang
- Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- David Battaglia
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Thomas O’Leary
- Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, 3303 Southwest, Bond Avenue
- Sacha A. Krieg
- Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, 3303 Southwest, Bond Avenue
- David M. Lee
- Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, 3303 Southwest, Bond Avenue
- Diana H. Wu
- Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, 3303 Southwest, Bond Avenue
- P. Barton Duell
- Knight Cardiovascular Institute, Oregon Health & Science University, 3181 Southwest, Sam Jackson Park Road
- Sanjiv Kaul
- Knight Cardiovascular Institute, Oregon Health & Science University, 3181 Southwest, Sam Jackson Park Road
- Jin-Soo Kim
- Center for Genome Engineering, Institute for Basic Science, 70, Yuseong-daero 1689-gil, Yuseong-gu
- Stephen B. Heitner
- Knight Cardiovascular Institute, Oregon Health & Science University, 3181 Southwest, Sam Jackson Park Road
- Eunju Kang
- Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University
- Zi-Jiang Chen
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Paula Amato
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- Shoukhrat Mitalipov
- Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University
- DOI
- https://doi.org/10.1038/s41467-023-36820-6
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 15
Abstract
DNA repair in response to DSBs in the preimplantation embryo is hard to analyze. Here the authors show that over 25% of pre-existing heterozygous loci in control single blastomere samples appeared as homozygous after whole genome amplification, therefore, they validated gene editing seen in human embryos in ESCs.
