Use of placenta cryoextract for restoring the morphofunctional state of reproductive organs in animals with syndrome of premature ovarian insufficiency. Experimental substantiation

Abstract

Objective of the study was to determine the placenta cryoextract impact on the restoration of the morphofunctional ability of the BALB/c mice uterus and ovaries after modeling their premature ovarian failure syndrome (POF) in the experiment. Materials and methods. A model of POF was created in 60 BALB/c mice which were divided into 2 groups of 30 animals: group 1 – with a model of POF without treatment, group 2 – with a model of POF and treatment with a placental cryoextract. The 3 (control) group included 30 intact BALB/c mice. Results. 4 weeks after creation of experimental POF in animals of group 1 histological examination of ovary showed an increase in interstitial tissue, replacing the generative structures of the ovary. An increased number of fibroblast cell elements and collagen fibers were also shown, indicating the growth of the organ stroma. Weight dropped sharply to 17.5 g, followed by a slow recovery in all animals of groups 1 and 2 after reproduction of the POF model. In this case, the weight in animals of group 2corresponded to the indices of control group already at 5th week and amounted to 21 g, reaching 22.1 g at 8th week. Animals in the 1 group restored their weight to 21.1 g only at 8th week. 5 weeks after POF simulation a complete restoration of estrous cycles occurred in 20% of in group 1 and in 70% in group 2. At week 8th the number of effective mating in group 1 was only 30%, while in group 2 this indicator increased to 80%. Conclusion. Intraperitoneal administration of cryopreserved placenta extract in mice with experimentally simulated POF leads to restoration of uterus morphological structure and partial restoration of ovarian structure with appearance of follicle-like generative elements in them.

Keywords

• experimental model
• premature ovarian failure
• placenta cryoextract.