Clinical, Cosmetic and Investigational Dermatology (May 2022)
The Role of Recombinant Secretory Leukocyte Protease Inhibitor to CD163, FGF-2, IL-1 and IL-6 Expression in Skin Wound Healing
Abstract
Elly Munadziroh,1 Giovani Anggasta Putri,2 Vera Ristiana,2 Titien Hary Agustantina,1 Intan Nirwana,1 Fathilah Abdul Razak,1,3 Meircurius Dwi Condro Surboyo4 1Department of Dental Materials, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia; 2Bachelor of Dental Sciences, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia; 3Department of Oral and Craniofacial Sciences, Faculty of Dentistry, Universiti Malaya, Kuala Lumpur, 50603, Malaysia; 4Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, IndonesiaCorrespondence: Elly Munadziroh, Department of Dental Materials, Faculty of Dental Medicine, Universitas Airlangga, Jalan Prof. Dr. Moestopo No. 47, Surabaya, 60132, Indonesia, Tel +6231-5030255, Email [email protected]: The wound healing process can be optimized through the addition of a biomaterial such as recombinant secretory leukocyte protease inhibitor (rSLPI). The SLPI is a non-glycosylated proteomic material that inhibits protease enzymes and has anti-inflammatory properties, thus accelerating wound healing. This study analyzed the administration of rSLPI doses 0.04 cc and 0.06 cc in skin wound healing on the CD163 expression of macrophages and cytokines such as interleukin 1 (IL-1), interleukin 6 (IL-6) and fibroblast growth factor 2 (FGF-2).Materials and Methods: rSLPI produced from Escherichia coli TOP10 as the cloning host, BL21 (DE3) strains as the expression host and pET30a plasmids were used for the expression system construction. The wound was created on Wistar rat dorsal skin, then rSLPI 0.04 cc and 0.06 cc was administered. In the next four days, the back skin was biopsied and stained by immunohistochemistry to analyze the CD163, FGF-2, IL-1 and IL-6 expression.Results: The administration of rSLPI increased CD163 and FGF-2 expression dependent on dose (p< 0.05). On the other hand, administration of rSLPI decreased IL-1 and IL-6 expression depending on dose (p < 0.05).Conclusion: The administration of rSLPI is able to accelerate the wound healing process by increasing the CD163 and FGF-2 expression. The cytokines such as IL-1 and IL-6 decreased depending on rSLPI doses.Keywords: recombinant secretory leukocyte protease inhibitor, wound healing, skin
