CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome
Li Hao,
Qin Ruiying,
Liu Xiaoshuang,
Liao Shengxiang,
Xu Rongfang,
Yang Jianbo,
Wei Pengcheng
Affiliations
Li Hao
Key Laboratory of Rice Genetics & Breeding, Institute of Rice Research, Anhui Academy of Agricultural Science, Hefei 230031, China
Qin Ruiying
Key Laboratory of Rice Genetics & Breeding, Institute of Rice Research, Anhui Academy of Agricultural Science, Hefei 230031, China
Liu Xiaoshuang
Key Laboratory of Rice Genetics & Breeding, Institute of Rice Research, Anhui Academy of Agricultural Science, Hefei 230031, China
Liao Shengxiang
Key Laboratory of Rice Genetics & Breeding, Institute of Rice Research, Anhui Academy of Agricultural Science, Hefei 230031, China
Xu Rongfang
Key Laboratory of Rice Genetics & Breeding, Institute of Rice Research, Anhui Academy of Agricultural Science, Hefei 230031, China
Yang Jianbo
Corresponding authors.; Key Laboratory of Rice Genetics & Breeding, Institute of Rice Research, Anhui Academy of Agricultural Science, Hefei 230031, China
Wei Pengcheng
Corresponding authors.; Key Laboratory of Rice Genetics & Breeding, Institute of Rice Research, Anhui Academy of Agricultural Science, Hefei 230031, China
SUMMARY: Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor (ABE)-nCas9 tool that induced targeted A·T to G·C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.
