Инновационная медицина Кубани (Nov 2024)

Modeling of Healing of Intra-Articular Fractures of Long Tubular Bones in Rats

  • D. A. Zinovkin,
  • V. I. Nikolaev,
  • N. V. Chueshova,
  • N. N. Veyalkina,
  • P. Yu. Ignatenko,
  • V. V. Malyukov,
  • M. Z.I. Pranjol,
  • E. S. Zinovkina

DOI
https://doi.org/10.35401/2541-9897-2024-9-4-77-84
Journal volume & issue
Vol. 0, no. 4
pp. 77 – 84

Abstract

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Background: We present a new original method of modeling an intra-articular fracture of the long tubular bone in laboratory animals.Objective: To create a new experimental model of an intra-articular fracture of the long tubular bone and to approve it for comprehensive evaluation of morphofunctional parameters of cartilage and bone healing.Materials and methods: The experiment was performed on 30 white sexually mature Wistar rats. Modeling involved layer-by-layer dissection of tissues along the anteromedial surface and formation of an incomplete fracture of the femoral medial condyle using a sharp L-shaped working end of an 0.2 × 0.2 сm instrument perpendicular to the medial condyle with a slight single muscle force. The rats were euthanized in groups of 10 animals on days 7, 14, and 30. We performed knee radiography and determined blood osteocalcin and BMP-7 levels. The Mankin score was used in the histological evaluation of the articular cartilage changes. The groups were compared using the Kruskal-Wallis test. P < .05 was considered statistically significant.Results: We noted statistically significant differences (P < .0001) when comparing the groups using the Mankin score. The post hoc test revealed statistically significant differences in the Mankin scores between days 7 and 14 (P = .014) and between days 7 and 30 (P < .0001). Group comparisons showed no statistically significant differences in blood osteocalcin levels (P = .518). However, there were statistically significant differences (P = .0028) between the groups in blood BMP-7 levels. The post hoc test revealed statistically significant differences in blood BMP-7 levels between days 7 and 14 (P = .048) and between days 14 and 30 (P = .0195).Conclusions: The developed experimental model of intra-articular fractures of long tubular bones allows for a comprehensive assessment of reparative processes of cartilage and bone tissue. The model is close to the real mechanism of pathology development.

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