Xanthine oxidase, α-glucosidase and α-amylase inhibitory activities of the essential oil from Piper lolot: In vitro and in silico studies
Tan Khanh Nguyen,
Linh Thuy Thi Tran,
Duc Ho Viet,
Pham Hong Thai,
Tran Phuong Ha,
Pham Viet Ty,
Le Phu Duc,
Dat Ton That Huu,
Le Canh Viet Cuong
Affiliations
Tan Khanh Nguyen
Institute of Applied Life Sciences, Dong A University, 33 Xo Viet Nghe Tinh, Hai Chau District, Da Nang, Viet Nam; Scientific Management Department, Dong A University, Da Nang, Viet Nam
Linh Thuy Thi Tran
Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen, Hue City, Thua Thien Hue, Viet Nam
Duc Ho Viet
Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen, Hue City, Thua Thien Hue, Viet Nam
Pham Hong Thai
Mientrung Institute for Scientific Research, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 321 Huynh Thuc Khang, Hue City, Thua Thien Hue, Viet Nam
Tran Phuong Ha
Mientrung Institute for Scientific Research, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 321 Huynh Thuc Khang, Hue City, Thua Thien Hue, Viet Nam
Pham Viet Ty
Faculty of Chemistry, University of Education, Hue University, 34 Le Loi, Hue City, Thua Thien Hue, Viet Nam
Le Phu Duc
Hue Medical College, People's Committee of Thua Thien Hue Province, 01 Nguyen Truong to, Hue City, Thua Thien Hue, Viet Nam
Dat Ton That Huu
Mientrung Institute for Scientific Research, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 321 Huynh Thuc Khang, Hue City, Thua Thien Hue, Viet Nam; Corresponding author.
Le Canh Viet Cuong
Mientrung Institute for Scientific Research, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 321 Huynh Thuc Khang, Hue City, Thua Thien Hue, Viet Nam; Centre for Conservation of Vietnam Natural Resources and Rescue of Animals and Plants, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, Phong My, Phong Dien, Thua Thien Hue, Viet Nam; Corresponding author. Mientrung Institute for Scientific Research, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 321 Huynh Thuc Khang, Hue city, Thua Thien Hue, Viet Nam.
Introduction: Piper lolot is a species of herb used as a popular food in Vietnam. Furthermore, the species has been used as a Vietnamese traditional medicine to treat many diseases. Methods: Chemical constituents in the essential oil from leaves of Piper lolot were determined using GC/MS analysis. The anti-gout and anti-diabetic activities of the essential oil were determined through the inhibitory assays against xanthine oxidase, α-glucosidase and α-amylase enzymes. In addition, molecular docking simulations were used to elucidate the inhibitory mechanism between the main compounds and the enzymes. Results: The dominant constituents of the Piper lolot essential oils were determined as β-caryophyllene (20.6%), β-bisabolene (11.6%), β-selinene (8.4%), β-elemene (7.7%), trans-muurola-4(14),5-diene (7.4%), and (E)-β-ocimene (6.7%). The essential oil displayed xanthine oxidase, α-amylase, and α-glucosidase inhibitory activities with IC50 values of 28.4, 130.6, and 59.1 μg/mL, respectively. The anti-gout and anti-diabetic activities of the essential oil from the P. lolot species are reported for the first time. Furthermore, molecular docking simulation was consistent to in vitro experiments. Conclusion: The present study provides initial evidence that the essential oil of P. lolot may be a potential natural source to develop new diabetes preparations.
