Antifungal activity of silver/silicon dioxide nanocomposite on the response of faba bean plants (Vicia faba L.) infected by Botrytis cinerea

Abstract

Abstract Silicon (Si) and its nanomaterials could help plants cope with different negative effects of abiotic and/or biotic stresses. In this study, the antifungal role of silver/silicon dioxide nanocomposite (Ag/SiO2NC) biosynthesized using a free-cell supernatant of Escherichia coli D8 was investigated for controlling the growth parameters and yield of faba bean (Vicia faba L.) infected by Botrytis cinerea. This nanocomposite was characterized using UV–Vis spectroscopy, Fourier transform-infrared (FTIR), transmission electron microscopy (TEM), zeta analysis, and X-ray diffraction pattern (XRD). Positively charged Ag/SiO2NC (+ 31.0 mV) with spherical-shaped silver nanoparticles (AgNPs) showed strong in vitro antifungal activity with minimal inhibition concentration (MIC) value equal to 40 ppm. In vivo experiments revealed the good resistance of Ag/SiO2NC-treated plants against the B. cinerea infection due to the increase of total phenolic content, peroxidase, and polyphenol oxidase activity. The ultrastructure of Ag/SiO2NC-treated plants showed normal morphology of cells including cell membranes and ellipsoidal-shaped chloroplasts with big starch grains. The concentration of silver content in Ag/SiO2NC-treated plants was similar to the untreated control plant indicating the low realizability of AgNPs. All of these results are promising outcomes for the application of the biosynthesized Ag/SiO2NC as a safe and effective antifungal agent against B. cinerea. Graphical Abstract

Keywords

• Antifungal activity
• Silver
• Silicon dioxide
• Nanocomposite
• Vicia faba
• Botrytis cinerea