Shiyan dongwu yu bijiao yixue (Aug 2021)
Mechanism of Hypertonic Saline to Reduce Cerebral Edema in Rats with Cerebral Hemorrhage by Protecting Blood-brain Barrier
Abstract
ObjectiveTo investigate the mechanism of hypertonic saline (HS) treatment to reduce cerebral edema in intracerebral hemorrhage (ICH) rats by.MethodsSixty male SD rats were randomly divided into control, model, and treatment groups. An ICH rat model was established by injecting fetal bovine serum (FBS) into the brain parenchyma of rats. The rats in the treatment group were injected with 0.3 mL/h 10% HS via the tail vein for 48 h. The mortality of the rats was compared between the groups. Brain tissues were collected to observe the morphological and pathological changes, detect the water content differences in the brain, and measure the ratio of matrix metalloproteinase-9 (MMP-9) to tissue inhibitor of metalloproteinases-1 (TIMP-1) and the expression of related tight junction proteins, including claudins, occludin, and zonula occludens-1 (ZO-1).ResultsThe mortality and water content of the brain tissues in the model group were significantly higher than those in the treatment group (all P < 0.05). The naked eye observation showed that the midline of the brain in the model group shifted from the lesion side to the contralateral side, while the midline of the brain in the treatment group did not. The ratio of MMP-9/TIMP-1 in the brain tissue of rats in the treatment group was significantly lower than that in the model group, while the tight junction proteins of claudin-5, occludin, and ZO-1 in the brain tissue of rats in the treatment group were higher than those in the model group (all P < 0.05).ConclusionHS can maintain the structural integrity of the blood-brain barrier by reducing the ratio of MMP-9/TIMP-1, stably and tightly connecting the expression of claudin-5, occludin, and ZO-1, thereby improving the severity of brain edema after ICH and reducing the mortality of experimental model rats.
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