SURVIVAL OF LACTOBACILLUS PLANTARUM ONU 12 IN SOIL

Abstract

Aim. To reveal the terms of survival of bacteria Lactobacillus plantarum ONU 12 in soil. Materials and Methods. Bacterial culture of the strain L. plantarum ОNU 12 was added to chernozem and peat soil in two variants – without plants and with plants Kalanchoedaigremontiana Mill to reach the concentration1x108 CFU/g. Every four days the amount of lactobacilli was evaluated by inoculationon agarized MRS medium, and by study of phenotypic characteristics and further identification by polymerase chain reaction. Results. Lactobacilli survived for the longest period in plant rhizosphere in humus: on the 44thday of experiment their amount was (2.8±0.4)х102 CFU/g, and in humus without plants they survived at least for 36 days. In peatsoil with outplants lactobacilli survived for the shortest period, and they could not be isolated after 32th day. In rhizosphere of plants in peat soil they survived at least for 36 days. Conclusion. Depending on soil type and presence of plants, lactobacilli are able to survive up to 44 days.

Keywords

• молочнокислі бактерії
• ґрунт
• ризосфера
• збереження життєздатності