Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle
Korakrit Poonsuk,
Carita Kordik,
Matthew Hille,
Ting-Yu Cheng,
William B. Crosby,
Amelia R. Woolums,
Michael L. Clawson,
Carol Chitko-McKown,
Bruce Brodersen,
John Dustin Loy
Affiliations
Korakrit Poonsuk
Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USA
Carita Kordik
Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USA
Matthew Hille
Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USA
Ting-Yu Cheng
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA
William B. Crosby
Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762, USA
Amelia R. Woolums
Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762, USA
Michael L. Clawson
United States Department of Agriculture (USDA), Agricultural Research Service (ARS), United States Meat Animal Research Center, Clay Center, NE 68933, USA
Carol Chitko-McKown
United States Department of Agriculture (USDA), Agricultural Research Service (ARS), United States Meat Animal Research Center, Clay Center, NE 68933, USA
Bruce Brodersen
Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USA
John Dustin Loy
Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USA
Mannheimia haemolytica is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using M. haemolytica A1 strain P1148. In this study, the ELISAs were initially developed using sera from both M. haemolytica-culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status M. haemolytica sera (n = 145) collected from feedlot cattle. The test showed good inter-assay and intra-assay repeatability. Diagnostic sensitivity and specificity were estimated at 91% and 87% for IgG at a cutoff of S/P ≥ 0.8. IgM diagnostic sensitivity and specificity were 91% and 81% at a cutoff of sample to positive (S/P) ratio ≥ 0.8. IgA diagnostic sensitivity was 89% whereas specificity was 78% at a cutoff of S/P ≥ 0.2. ELISA results of all isotypes were related to the diagnosis of respiratory disease and isolation of M. haemolytica (p-value M. haemolytica ELISAs can be adapted to the detection and quantification of antibody in serum specimens and support the use of these tests for the disease surveillance and disease prevention research in feedlot cattle.
