Development and Genome Sequencing of a Laboratory-Inbred Miniature Pig Facilitates Study of Human Diabetic Disease
Li Zhang,
Yuemeng Huang,
Meng Wang,
Yafen Guo,
Jing Liang,
Xiurong Yang,
Wenjing Qi,
Yanjun Wu,
Jinglei Si,
Siran Zhu,
Zhe Li,
Ruiqiang Li,
Chao Shi,
Shuo Wang,
Qunjie Zhang,
Zhonglin Tang,
Lixian Wang,
Kui Li,
Ji-Feng Fei,
Ganqiu Lan
Affiliations
Li Zhang
College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Yuemeng Huang
College of Animal Science and Technology, Guangxi University, Nanning 530004, China; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
Meng Wang
Novogene Bioinformatics Institute, Beijing 100083, China
Yafen Guo
College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Jing Liang
College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Corresponding author
Xiurong Yang
College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Wenjing Qi
College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Yanjun Wu
College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Jinglei Si
College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Siran Zhu
College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Zhe Li
Novogene Bioinformatics Institute, Beijing 100083, China
Ruiqiang Li
Novogene Bioinformatics Institute, Beijing 100083, China
Chao Shi
Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; Corresponding author
Shuo Wang
Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Qunjie Zhang
Institution of Genomics and Bioinformatics, South China Agricultural University, Guangzhou 510642, China
Zhonglin Tang
Research Centre for Animal Genome, Agricultural Genome Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China; Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genome Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China
Lixian Wang
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Kui Li
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Ji-Feng Fei
Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou 510631, China
Ganqiu Lan
College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Corresponding author
Summary: Pig has been proved to be a valuable large animal model used for research on diabetic disease. However, their translational value is limited given their distinct anatomy and physiology. For the last 30 years, we have been developing a laboratory Asian miniature pig inbred line (Bama miniature pig [BM]) from the primitive Bama xiang pig via long-term selective inbreeding. Here, we assembled a BM reference genome at full chromosome-scale resolution with a total length of 2.49 Gb. Comparative and evolutionary genomic analyses identified numerous variations between the BM and commercial pig (Duroc), particularly those in the genetic loci associated with the features advantageous to diabetes studies. Resequencing analyses revealed many differentiated gene loci associated with inbreeding and other selective forces. These together with transcriptome analyses of diabetic pig models provide a comprehensive genetic basis for resistance to diabetogenic environment, especially related to energy metabolism. : Biological Sciences; Gene Ontology; Genomic Analysis; Genomics; Omics; Sequence Analysis; Transcriptomics Subject Areas: Biological Sciences, Gene Ontology, Genomic Analysis, Genomics, Omics, Sequence Analysis, Transcriptomics
