Nature Communications (Jul 2019)

Lysine/RNA-interactions drive and regulate biomolecular condensation

  • Tina Ukmar-Godec,
  • Saskia Hutten,
  • Matthew P. Grieshop,
  • Nasrollah Rezaei-Ghaleh,
  • Maria-Sol Cima-Omori,
  • Jacek Biernat,
  • Eckhard Mandelkow,
  • Johannes Söding,
  • Dorothee Dormann,
  • Markus Zweckstetter

DOI
https://doi.org/10.1038/s41467-019-10792-y
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 15

Abstract

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Processing bodies (P-bodies) are non-membrane-bound protein/RNA granules in the cytosol. Here the authors combine bioinformatics, NMR and cell based assays and find that lysine is enriched in the disordered regions of P-body-associated proteins and show that lysine-rich polypeptides form highly dynamic lysine/RNA-coacervates and lysine acetylation reverses liquid-liquid phase separation.