The in situ remediation of aquaculture water and sediment by commercial probiotics immobilized on different carriers

Abstract

In the present study, we investigated the effect of probiotics immobilized by oyster shells (Os), vesuvianite (Ve) and walnut shells (Ws) on the remediation of aquaculture water and sediment by analyzing the variation of ammonia-nitrogen (NH4–N), nitrate-nitrogen (NO3–N), nitrite-nitrogen (NO2–N), total nitrogen (TN), total phosphorus (TP) and chemical oxygen demand (CODCr), as well as the microbiota of the water and sediment. The positive or negative effects of the treatment groups on the water quality parameters were both observed. Compared with their effects on water quality parameters, the treatment groups had better effects on sediment parameters. Group Ve had the best remediation effect of NH4–N and NO3–N in the sediment (decreased by 5.22 and 1.66 times, respectively). Group Os showed a lower relative concentration of TN and CODCr (decreased by 3.77 and 0.95 times, respectively). The high-throughput sequencing results revealed that the immobilized probiotics increased the relative abundances of functional bacteria in the treatment groups at the phylum and genus level. The above results showed that probiotics immobilized by oyster shells, vesuvianite and walnut shells positively affected the aquaculture environment's remediation, especially the sediment. HIGHLIGHTS The effects of probiotics immobilized by oyster shells, vesuvianite and walnut shells on the remediation of aquaculture water and sediment were researched.; The effect of immobilized probiotics on nutrient content and microbiota in the water and sediment was tested.; Probiotics immobilized by oyster shells, vesuvianite and walnut shells affect the remediation of aquaculture water and sediment.;

Keywords

• aquaculture water
• immobilized probiotic
• in situ remediation
• microbial community