Antimicrobial Susceptibility and Genomic Profiles of Multidrug-Resistant <i>Staphylococcus aureus</i> from Nasopharynx of Asymptomatic Children in Dhaka, Bangladesh
Sufia Islam,
Nishat Nasrin,
Nigar Sultana Tithi,
Farjana Khatun,
Muhammad Asaduzzaman,
Anika Fatema Topa,
Md Farhad Kabir,
Fahim Kabir Monjurul Haque,
Mohammad Jubair,
Mustafizur Rahman,
Christian Lehmann
Affiliations
Sufia Islam
Department of Pharmacy, East West University, Dhaka 1212, Bangladesh
Nishat Nasrin
Department of Pharmacy, East West University, Dhaka 1212, Bangladesh
Nigar Sultana Tithi
Department of Pharmacy, East West University, Dhaka 1212, Bangladesh
Farjana Khatun
Department of Pharmacy, East West University, Dhaka 1212, Bangladesh
Muhammad Asaduzzaman
Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka 1000, Bangladesh
Anika Fatema Topa
Department of Pharmacy, East West University, Dhaka 1212, Bangladesh
Md Farhad Kabir
Nutrition & Clinical Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka 1212, Bangladesh
Fahim Kabir Monjurul Haque
Department of Mathematics and Natural Sciences, BRAC University, Dhaka 1212, Bangladesh
Mohammad Jubair
Genome Centre, Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka 1212, Bangladesh
Mustafizur Rahman
Genome Centre, Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka 1212, Bangladesh
Christian Lehmann
Department of Anesthesia, Pain Management and Perioperative Medicine, Faculty of Medicine, Dalhousie University, Halifax, NS B3H 1X5, Canada
Children carrying Staphylococcus aureus in their nasopharynx are at a higher risk of contracting systemic infection. Due to lack of sufficient information regarding such carriage, this study was conducted to explore the prevalence, antibiotic susceptibility, and genomic profiles of S. aureus isolated from nasopharyngeal samples of 163 randomly selected asymptomatic Bangladeshi children aged from 5–p = 0.002) and influenza (p = 0.004). Among the isolates, 84.1% were multidrug-resistant and 47.5% (n = 40) were methicillin-resistant S. aureus (MRSA). All the isolates (100%) were resistant to cefixime with higher resistance to ampicillin (95.5%) and penicillin (90.9%). Among the three investigated isolates, two were ST80 (ID-1 and ID-52) and one was a novel strain (ID-19) with the presence of aph-Stph, blaI, blaZ, dha1, fosB, lmrS, mepA, norA, and tet38 genes. The current research demonstrates a high incidence of multidrug-resistant S. aureus and reports the first instance of ST80 in asymptomatic children in Bangladesh.
