A New Circular Single-Stranded DNA Virus Related with Howler Monkey Associated Porprismacovirus 1 Detected in Children with Acute Gastroenteritis
Fabiola Villanova,
Flávio Augusto de Padua Milagres,
Rafael Brustulin,
Emerson Luiz Lima Araújo,
Ramendra Pati Pandey,
V. Samuel Raj,
Xutao Deng,
Eric Delwart,
Adriana Luchs,
Antonio Charlys da Costa,
Élcio Leal
Affiliations
Fabiola Villanova
Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, Pará, Brazil
Flávio Augusto de Padua Milagres
Secretary of Health of Tocantins, Palmas 77453-000, Tocantins, Brazil
Rafael Brustulin
Secretary of Health of Tocantins, Palmas 77453-000, Tocantins, Brazil
Emerson Luiz Lima Araújo
General Coordination of Public Health Laboratories of the Strategic Articulation Department of the Health Surveillance Secretariat of the Ministry of Health (CGLAB/DAEVS/SVS-MS), Brasília 70719-040, Distrito Federal, Brazil
Ramendra Pati Pandey
Centre for Drug Design Discovery and Development (C4D), SRM University, Delhi-NCR, Rajiv Gandhi Education City, Sonepat 131029, Haryana, India
V. Samuel Raj
Centre for Drug Design Discovery and Development (C4D), SRM University, Delhi-NCR, Rajiv Gandhi Education City, Sonepat 131029, Haryana, India
Xutao Deng
Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118, USA
Eric Delwart
Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118, USA
Adriana Luchs
Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo 01246-000, São Paulo, Brazil
Antonio Charlys da Costa
Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, São Paulo, Brazil
Élcio Leal
Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, Pará, Brazil
Putative replication-associated protein (REP) and capsid-like (CAP) proteins are encoded by circular single-stranded DNA viruses (CRESS DNA), which have been found in samples from most eukaryotic groups. However, the details of these viruses’ life cycles and their significance in diseases have yet to be established. We presented and analyzed two full-length CRESS DNA genomes acquired from two children diagnosed with acute gastroenteritis (GI) in the northeast state of Tocantins, Brazil, using next-generation sequencing and a virus-like filtration approach. Both sequences (named SmaCV3BR08 and SmaCV3BR291) are closely similar to a prior CRESS DNA sequence discovered in the feces of a new world monkey (Alouatta caraya) from the United States in 2009 and termed Howler monkey-associated porprismacovirus 1 (Genbank ID: NC 026317). According to our comparative study, these porprismacovirus genomes deviate by 10% at the nucleotide level. For comparative reasons, the divergence between our sequences (SmaCV3BR08 and SmaCV3BR291) and a porprismacovirus recently identified in a human fecal sample from Peru is 37%. These data suggest that there is a great diversity of porprismacoviruses in South America, perhaps more than two species. In addition, the finding of closely related sequences of porprismacoviruses in humans and native monkeys highlights the zoonotic potential of these viruses.
