Scientific Reports (Jul 2017)

Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks

  • Michael Ansorge,
  • Jiranuwat Sapudom,
  • Marina Chkolnikov,
  • Martin Wilde,
  • Ulf Anderegg,
  • Stephanie Möller,
  • Matthias Schnabelrauch,
  • Tilo Pompe

DOI
https://doi.org/10.1038/s41598-017-05912-x
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 8

Abstract

Read online

Abstract TGFβ1 is a key regulator for induction of tissue remodeling after dermal wounding. We present a model of paracrine delivery of TGFβ1 for differentiation of dermal fibroblasts based on a fibrillar 3D collagen matrix and embedded TGFβ1 releasing microparticles. We found differentiation into myofibroblasts was achieved in a TGFβ1 dependent manner at much lower doses than systemic delivery. This effect is accounted to the slow and sustained TGFβ1 release mimicking paracrine cell signals.