Research Institute of Internal and Preventive Medicine — Branch of Federal Research Center Institute of Citology and Genetics of SB RAS Russian Federation
Research Institute of Internal and Preventive Medicine — Branch of Federal Research Center Institute of Citology and Genetics of SB RAS Russian Federation
V. P. Novoselov
Novosibirsk State Medical University of Minzdrav of Russia Russian Federation
I. A. Rodina
ГБУЗ НСО Новосибирское областное клиническое бюро судебно-медицинской экспертизы Russian Federation
O. V. Khamovich
ГБУЗ НСО Новосибирское областное клиническое бюро судебно-медицинской экспертизы Russian Federation
Research Institute of Internal and Preventive Medicine — Branch of Federal Research Center Institute of Citology and Genetics of SB RAS Russian Federation
The aim of the research is to verify the association with sudden cardiac death (SCD) of single nucleotide polymorphisms rs10867772 and rs4700290, identified as new molecular genetic markers of SCD in the own genome-wide pooled allelotyping. Material and methods. Case-control study. The SCD group is formed using the criteria of the European Society of Cardiology from the DNA bank of suddenly deceased residents of the Oktyabrsky district of Novosibirsk (n = 437, average age—53.1 ± 9.0 years, men — 73.5%, women — 26.5%) The control group (n = 405, average age 53.2 ± 9.2 years, men — 70.0%, women — 30.0%) is formed from the DNA bank of participants of MONICA and HAPIEE projects. DNA was isolated by phenol-chloroform extraction from myocardial tissue in the SCD group and venous blood in the control group. Genotyping was performed by the PCR-RFLP method. Results. No statistical significance was found in allele and genotype frequencies of rs10867772 and rs4700290 between groups, even in separating in sex and age (p> 0.05). Conclusion. Single nucleotide polymorphism rs10867772 and rs4700290 are not associated with SCD.
