Building in vitro 3D human multicellular models of high-grade serous ovarian cancer

Abstract

Summary: Three-dimensional (3D), multicellular in vitro models provide a useful platform for studying human cancer biology, particularly through deconvolution of the tumor microenvironment, or where animal models do not recapitulate the human condition. Here, we detail a protocol for building human multicellular models made of patient-derived primary cells and malignant cell lines, which recapitulate features of the tumor microenvironment. This protocol is optimized for building 3D models of high-grade serous ovarian cancer omental metastasis but can be adapted for modeling other cancers.For complete details on the use and execution of this profile, please refer to Delaine-Smith et al. (2021) and Malacrida et al. (2021).

Keywords

• Cancer
• Cell Biology
• Cell culture
• Cell isolation