Mouse Satellite Cell Isolation and Transplantation
Elia Angelino,
Simone Reano,
Michele Ferrara,
Emanuela Agosti,
Hana Sustova,
Valeria Malacarne,
Sara Clerici,
Andrea Graziani,
Nicoletta Filigheddu
Affiliations
Elia Angelino
Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM), Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Simone Reano
Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM)
Michele Ferrara
Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM), Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Emanuela Agosti
Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM)
Hana Sustova
Department of Translational Medicine, University of Piemonte Orientale, Novara, ItaliaIstituto Interuniversitario di Miologia (IIM)
Valeria Malacarne
Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyDivision of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Sara Clerici
Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Andrea Graziani
Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM), Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Nicoletta Filigheddu
Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM)
Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs (Reano et al., 2017).
