Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria

Abstract

A survey was conducted of central and western Algerian grape cultivars and a germplasm collection to detect the presence of Grapevine leafroll-associated virus 1 (GLRaV-1), using DAS-ELISA. The virus was found in 26 of 484 sample (5.4%). No infection was found in the germplasm collection. Analysis of the sequence of a coat protein gene region revealed that of the 17 recognised phylogenetic groups, the Algerian isolates belong to Groups I, II and XVI. This is the first study of genetic diversity of GLRaV-1 in Algeria.

Keywords

• GLRaV-1
• RT-PCR
• genetic variability
• HSP70h
• CP