Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology

Maryam Alowaysi; Mohammad Al-Shehri; Moayad Baadhaim; Hajar AlZahrani; Doaa Aboalola; Mustafa Daghestani; Heba Hashem; Rawan Aljahdali; Rayan Salem; Adel Alharbi; Mohammed Muharraq; Khaled Alghamdi; Fawaz Alsobiy; Asima Zia; Robert Lehmann; Jesper Tegner; Khaled Alsayegh

Stem Cell Research (Sep 2023)

Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology

Maryam Alowaysi,
Mohammad Al-Shehri,
Moayad Baadhaim,
Hajar AlZahrani,
Doaa Aboalola,
Mustafa Daghestani,
Heba Hashem,
Rawan Aljahdali,
Rayan Salem,
Adel Alharbi,
Mohammed Muharraq,
Khaled Alghamdi,
Fawaz Alsobiy,
Asima Zia,
Robert Lehmann,
Jesper Tegner,
Khaled Alsayegh

Affiliations

Maryam Alowaysi: King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia
Mohammad Al-Shehri: King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia
Moayad Baadhaim: King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia
Hajar AlZahrani: King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia
Doaa Aboalola: King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia
Mustafa Daghestani: King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia; Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia
Heba Hashem: Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia
Rawan Aljahdali: Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia
Rayan Salem: Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia
Adel Alharbi: Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia
Mohammed Muharraq: Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia
Khaled Alghamdi: Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia
Fawaz Alsobiy: Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia
Asima Zia: Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Robert Lehmann: Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Jesper Tegner: Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Khaled Alsayegh: King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia; Corresponding author.

Journal volume & issue: Vol. 71
p. 103158

Abstract

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Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB’s role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB−/−) via CRISPR/Cas9 to study MB function in human biology and diseases.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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