Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions

Abstract

Nash et al. develop and characterise multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts, which constitute an accessible model of the chicken intestine. This model enables manipulations, such as viral infection, to be performed without the need for micro-injection, thus opening up more opportunities to explore epithelial and leukocyte interactions and responses in host–pathogen, food science and pharmaceutical research.