High-depth spatial transcriptome analysis by photo-isolation chemistry

Mizuki Honda; Shinya Oki; Ryuichi Kimura; Akihito Harada; Kazumitsu Maehara; Kaori Tanaka; Chikara Meno; Yasuyuki Ohkawa

doi:10.1038/s41467-021-24691-8

Nature Communications (Jul 2021)

High-depth spatial transcriptome analysis by photo-isolation chemistry

Mizuki Honda,
Shinya Oki,
Ryuichi Kimura,
Akihito Harada,
Kazumitsu Maehara,
Kaori Tanaka,
Chikara Meno,
Yasuyuki Ohkawa

Affiliations

Mizuki Honda: Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University
Shinya Oki: Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University
Ryuichi Kimura: Department of Drug Discovery Medicine, Kyoto University Graduate School of Medicine
Akihito Harada: Division of Transcriptomics, Medical Institute of Bioregulation, Kyushu University
Kazumitsu Maehara: Division of Transcriptomics, Medical Institute of Bioregulation, Kyushu University
Kaori Tanaka: Division of Transcriptomics, Medical Institute of Bioregulation, Kyushu University
Chikara Meno: Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University
Yasuyuki Ohkawa: Division of Transcriptomics, Medical Institute of Bioregulation, Kyushu University

DOI: https://doi.org/10.1038/s41467-021-24691-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 11

Abstract

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Spatial analysis of RNAseq data is important. Here the authors report a method for transcriptome profiling combined with photo-isolation chemistry to allow determination of expression profiles specifically from photo-irradiated regions of interest which they use in mouse brains and embryonic tissues.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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