Clinical, Cosmetic and Investigational Dermatology (Dec 2022)

Anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin Alleviates Ultraviolet-Induced Apoptosis of Human Skin Fibroblasts by Regulating the Death Receptor Pathway

  • Wang L,
  • Wan G,
  • Wang G,
  • Zhang M,
  • Li N,
  • Zhang Q,
  • Yan H

Journal volume & issue
Vol. Volume 15
pp. 2925 – 2932

Abstract

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Liwen Wang,1,* Guangmei Wan,1,* Gang Wang,1 Meihong Zhang,1 Nanxin Li,1 Qinning Zhang,2 Hualing Yan1 1Department of Dermatology, Qinghai University Affiliated Hospital, Xining, People’s Republic of China; 2Shijingshan Teaching Hospital, Capital Medical University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hualing Yan, Department of Dermatology, Qinghai University Affiliated Hospital, No. 29, Tongren Road, Chengxi District, Xining, Qinghai Province, People’s Republic of China, Email [email protected]: The study aimed to investigate the potential protective role of anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin against ultraviolet B (UVB)-induced apoptosis of human skin fibroblasts (HSFs).Methods: HSFs cultured in vitro were randomly divided into a control group, UVB group, and anthocyanin groups (0.1, 0.5, and 1.0 mg/mL). HSFs in the UVB and anthocyanin groups were exposed to 30 mJ/cm2 UVB to establish a photoaging model. Then, apoptosis rate, tumor necrosis factor-α (TNF-α), cysteinyl aspartate specific proteinase-3 (caspase-3), cysteinyl aspartate specific proteinase-7 (caspase-7), and survivin expression were evaluated.Results: UVB irradiation can increase the apoptosis rate of HSFs and expression of TNF-α, caspase-7, and survivin. Anthocyanin pretreatment (0.1, 0.5, and 1.0 mg/mL) decreased UVB-induced apoptosis rate and TNF-α and caspase-7 expression and increased survivin expression. Compared with the control group, the apoptosis rate and expression of TNF-α, caspase-7, and survivin of anthocyanin groups in UVB-irradiated HSFs were high. Among the three doses of anthocyanin (0.1, 0.5, and 1.0 mg/mL) groups, the apoptosis rate and TNF-α expression of anthocyanin at 1.0 mg/mL were the lowest. There was no significant change in caspase-3 expression in each group.Conclusion: Anthocyanin from Lycium ruthenicum Murr. in the Qaidam Basin could alleviate UVB-induced apoptosis by regulating the death receptor pathway.Keywords: Lycium ruthenicum Murr., anthocyanin, human skin fibroblasts, ultraviolet B, apoptosis

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