Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis

Abstract

Xiaolei Zhao,* Zhenhua Zhao,* Wenchao Xu, Hui Liu, Junkai Chang, Weibo Xu, Song Li, Songqiang Cao, Junqing Hou Department of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Junqing HouDepartment of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, People’s Republic of ChinaEmail [email protected]: Accumulating evidence points to a role for circular RNAs (circRNAs) in important regulatory function in tumor advancement. We explored the effect and function of circ-SAR1A in renal cell carcinoma (RCC).Methods: circ-SAR1A expression in RCC tissues and cell lines was explored by qRT-PCR. The roles of circ-SAR1A on RCC progression were explored by in vitro function assays. Moreover, we determined the underlying mechanism of circ-SAR1A in RCC progression through bioinformatics analysis and dual-luciferase reporter assays.Results: Our data reveal that circ-SAR1A is significantly high in RCC tissues and cell lines. High circ-SAR1A levels are correlated to advanced Fuhrman grade, and lymph-node metastasis in RCC patients. Functional experiments indicate that circ-SAR1A suppression decreased RCC cell growth and invasion abilities in vitro. Mechanistically, circ-SAR1A upregulated YBX1 expression by sponging miR-382, resulting in promoting the growth and invasion in RCC cells.Conclusion: Our data indicate that the circ-SAR1A/miR-382/YBX1 axis plays a critical role in RCC progression, which serve as a potential novel treatment strategy of RCC.Keywords: circ-SAR1A, miR-382, YBX1, renal cell carcinoma

Keywords

• circ-sar1a
• mir-382
• ybx1
• renal cell carcinoma