Frontiers in Immunology (Dec 2021)
Association of IL-9, IL-10, and IL-17 Cytokines With Hepatic Fibrosis in Human Schistosoma mansoni Infection
- Karine Garcez Schuster Franco,
- Karine Garcez Schuster Franco,
- Fabio Jorge Ramalho de Amorim,
- Fabio Jorge Ramalho de Amorim,
- Mário Adriano Santos,
- Carla Virgínia Vieira Rollemberg,
- Carla Virgínia Vieira Rollemberg,
- Fabricia Alvisi de Oliveira,
- Fabricia Alvisi de Oliveira,
- Alex Vianey Callado França,
- Alex Vianey Callado França,
- Camilla Natália Oliveira Santos,
- Camilla Natália Oliveira Santos,
- Lucas Sousa Magalhães,
- Lucas Sousa Magalhães,
- Rodrigo Anselmo Cazzaniga,
- Rodrigo Anselmo Cazzaniga,
- Frederico Santana de Lima,
- Luciana Benevides,
- Vanessa Carregaro,
- João Santana Silva,
- Hugo Leite de Farias Brito,
- Daniel Alvarenga Fernandes,
- Ângela Maria da Silva,
- Ângela Maria da Silva,
- Ângela Maria da Silva,
- Roque Pacheco de Almeida,
- Roque Pacheco de Almeida,
- Roque Pacheco de Almeida,
- Roque Pacheco de Almeida,
- Márcio Bezerra-Santos,
- Márcio Bezerra-Santos,
- Márcio Bezerra-Santos,
- Amélia Ribeiro de Jesus,
- Amélia Ribeiro de Jesus,
- Amélia Ribeiro de Jesus,
- Amélia Ribeiro de Jesus
Affiliations
- Karine Garcez Schuster Franco
- Image and Graphic Methods Unit, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Karine Garcez Schuster Franco
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Fabio Jorge Ramalho de Amorim
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Fabio Jorge Ramalho de Amorim
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Mário Adriano Santos
- Department of Medicine, University Hospital, Federal University of Sergipe, Aracaju, Brazil
- Carla Virgínia Vieira Rollemberg
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Carla Virgínia Vieira Rollemberg
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Fabricia Alvisi de Oliveira
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Fabricia Alvisi de Oliveira
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Alex Vianey Callado França
- Department of Medicine, University Hospital, Federal University of Sergipe, Aracaju, Brazil
- Alex Vianey Callado França
- Hepatology Service, University Hospital, Federal University of Sergipe, Aracaju, Brazil
- Camilla Natália Oliveira Santos
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Camilla Natália Oliveira Santos
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Lucas Sousa Magalhães
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Lucas Sousa Magalhães
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Rodrigo Anselmo Cazzaniga
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Rodrigo Anselmo Cazzaniga
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Frederico Santana de Lima
- Hepatology Service, University Hospital, Federal University of Sergipe, Aracaju, Brazil
- Luciana Benevides
- Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil
- Vanessa Carregaro
- Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil
- João Santana Silva
- Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil
- Hugo Leite de Farias Brito
- Division of Pathology, University Hospital, Federal University of Sergipe, Aracaju, Brazil
- Daniel Alvarenga Fernandes
- Image and Graphic Methods Unit, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Ângela Maria da Silva
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Ângela Maria da Silva
- Department of Medicine, University Hospital, Federal University of Sergipe, Aracaju, Brazil
- Ângela Maria da Silva
- Infectology Service, University Hospital, Federal University of Sergipe, Sergipe, Brazil
- Roque Pacheco de Almeida
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Roque Pacheco de Almeida
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Roque Pacheco de Almeida
- Department of Medicine, University Hospital, Federal University of Sergipe, Aracaju, Brazil
- Roque Pacheco de Almeida
- Immunology Institute of Investigation (III), National Institute of Science and Technology (INCT), Brazilian Research and Technology Council (CNPq), São Paulo, Brazil
- Márcio Bezerra-Santos
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Márcio Bezerra-Santos
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Márcio Bezerra-Santos
- 0Department of Morphology, Federal University of Sergipe, São Cristóvão, Brazil
- Amélia Ribeiro de Jesus
- Health Science Graduate Program, Federal University of Sergipe, Aracaju, Brazil
- Amélia Ribeiro de Jesus
- Laboratory of Molecular Biology, University Hospital, Federal Sergipe University, Aracaju, Brazil
- Amélia Ribeiro de Jesus
- Department of Medicine, University Hospital, Federal University of Sergipe, Aracaju, Brazil
- Amélia Ribeiro de Jesus
- Immunology Institute of Investigation (III), National Institute of Science and Technology (INCT), Brazilian Research and Technology Council (CNPq), São Paulo, Brazil
- DOI
- https://doi.org/10.3389/fimmu.2021.779534
- Journal volume & issue
-
Vol. 12
Abstract
This is a case series study to evaluate immunological markers associated with schistosomiasis advanced fibrosis, including 69 patients from an endemic area from the State of Sergipe and from the Hepatology Service of the University Hospital in Sergipe, Brazil. Hepatic fibrosis was classified based on Niamey protocol for ultrasonography (US). Immune response to Schistosoma mansoni antigens was evaluated by stimulating peripheral blood mononuclear cells (PBMCs) from these patients with either adult worm (SWAP—10 μg/ml) or egg (SEA—10 μg/ml) antigens or purified protein derivative of turberculin (PPD—10 μg/ml) or phytohemagglutinin (PHA—1 μg/ml) for 72 h. The levels of IFN-γ, TNF-α, IL-5, IL-10, and IL-17 were measured in these supernatants by ELISA and IL-9 by Luminex. Single nucleotide polymorphisms in IL-17, IL10, and CD209 genes were genotyped using TaqMan probe by qPCR. Higher levels of IL-9, IL-10, and IL-17 were found in PBMC supernatants of patients with advanced hepatic fibrosis. Direct correlations were detected between IL-9 and IL-17 levels with US spleen sizes, portal vein diameters, and periportal thickening. The CD209 rs2287886 AG polymorphism patients produce higher IL-17 levels. Together, these data suggest a role of these cytokines in the immunopathogenesis of advanced fibrosis in human schistosomiasis.
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