miR-344d-3p regulates osteogenic and adipogenic differentiation of mouse mandibular bone marrow mesenchymal stem cells
Wei Cao,
Xiaohong Yang,
Xiao Hua Hu,
Jun Li,
Jia Tian,
RenJun OuYang,
Xue Lin
Affiliations
Wei Cao
Department of Prosthodontics, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, China
Xiaohong Yang
Department of Prosthodontics, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, China
Xiao Hua Hu
Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, China
Jun Li
Department of Dental Implant, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, Guizhou, China, Zunyi, China
Jia Tian
Department of Prosthodontics, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, China
RenJun OuYang
Department of Prosthodontics, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, China
Xue Lin
Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi, China
Postmenopausal osteoporosis (POP) is a chronic disease of bone metabolism that occurs in middle-aged and elderly women. POP can cause abnormalities of the skeletal system in the whole body, and the jaw bone is also impacted, affecting the function of the oral and maxillofacial regions. Mandibular bone marrow mesenchymal stem cells (MBMSCs) play an important role in mandibular bone metabolism, and abnormal differentiation of MBMSCs can affect the metabolic balance between new and old bone. MicroRNAs (miRNAs) can induce the differentiation of MBMSCs. In this study, the changes in biological characteristics of mandible and MBMSCs in the bone microenvironment of postmenopausal osteoporosis were firstly analyzed, and then the key miRNAs screened from miRNAs gene chips were sorted out for verification and functional exploration. It was found that miR-344d-3p promoted the osteogenic differentiation of MC3T3-E1 and MBMSCs. It inhibited the adipogenic differentiation of 3T3-L1 and MBMSCs. In addition, Dnmt3a may be the target gene of miR-344d-3p. In conclusion, this study found new biological indicators related to bone metabolism, which are of great significance in the field of bone reconstruction.