Nature Communications (Dec 2022)

TAPE-seq is a cell-based method for predicting genome-wide off-target effects of prime editor

  • Jeonghun Kwon,
  • Minyoung Kim,
  • Seungmin Bae,
  • Anna Jo,
  • Youngho Kim,
  • Jungjoon K. Lee

DOI
https://doi.org/10.1038/s41467-022-35743-y
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

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Methods to predict genome-wide off-target activities of prime editors (PEs) are currently lacking. Here the authors report a cell-based assay, TAgmentation of Prime Editor sequencing (TAPE-seq), that provides genome-wide off-target candidates for PEs.