Characterization and Biological Evaluation of Propolis from Poland
Milena Popova,
Efstathia Giannopoulou,
Krystyna Skalicka-Woźniak,
Konstantia Graikou,
Jaroslaw Widelski,
Vassya Bankova,
Haralabos Kalofonos,
Gregory Sivolapenko,
Katarzyna Gaweł-Bęben,
Beata Antosiewicz,
Ioanna Chinou
Affiliations
Milena Popova
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
Efstathia Giannopoulou
Clinical Oncology Laboratory, University Hospital of Patras, Patras Medical School, 26504 Rio, Greece
Krystyna Skalicka-Woźniak
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 20093 Lublin, Poland
Konstantia Graikou
Division of Pharmacognosy and Chemistry of Natural Products, Departments of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece
Jaroslaw Widelski
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 20093 Lublin, Poland
Vassya Bankova
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
Haralabos Kalofonos
Clinical Oncology Laboratory, University Hospital of Patras, Patras Medical School, 26504 Rio, Greece
Gregory Sivolapenko
Pharmacokinetics Laboratory, Department of Pharmacy, University of Patras, 26504 Rio, Greece
Katarzyna Gaweł-Bęben
Department of Cosmetology, University of Information Technology and Management in Rzeszow, 35225 Rzeszów, Poland
Beata Antosiewicz
Department of Cosmetology, University of Information Technology and Management in Rzeszow, 35225 Rzeszów, Poland
Ioanna Chinou
Division of Pharmacognosy and Chemistry of Natural Products, Departments of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece
In this study, we assessed the therapeutic potential of propolis from Poland and performed chemical analysis by GC–MS, as well as determined its botanical origin. Chemical constituents typical for bud exudates of Populus nigra (section Aigeiros) were determined, however, glycerol esters of phenolic acids, as well as unusually high amounts of p-coumaric and ferulic acid and their benzyl esters, were also detected. These constituents are characteristic for buds of Populus tremula (section Leuce). We also evaluated the antiproliferative effect of propolis extracts against nine human cancer cell lines. Additionally, promising antibacterial activity of the dichloromethane extract (Minimal Inhibitory Concentration MIC values of 0.95–1.24 mg/mL), as well as a moderate antifungal activity (MIC values of 1.25–1.40 mg/mL), was noticed. Propolis from Poland appeared as a rich source of antibacterial and antiproliferative compounds and this confirmed that it is a valuable natural product with the potential to improve human health.
