Nature Communications (Oct 2022)

Rapid joule heating improves vitrification based cryopreservation

  • Li Zhan,
  • Zonghu Han,
  • Qi Shao,
  • Michael L. Etheridge,
  • Thomas Hays,
  • John C. Bischof

DOI
https://doi.org/10.1038/s41467-022-33546-9
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 15

Abstract

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Lower concentrations of toxic cryoprotective agents require rapid and scalable rewarming techniques. Here, the authors report on a joule heating–based platform where samples are rapidly rewarmed by contact with a voltage pulsed electrical conductor and demonstrate the preservation of Drosophila embryos and rat kidney slices.