Effects of <i>Lactobacillus rhamnosus</i> DSM7133 on Intestinal Porcine Epithelial Cells

Abstract

Antimicrobial resistance is one of the biggest health challenges nowadays. Probiotics are promising candidates as feed additives contributing to the health of the gastrointestinal tract. The beneficial effect of probiotics is species/strain specific; the potential benefits need to be individually assessed for each probiotic strain or species. We established a co-culture model, in which gastrointestinal infection was modeled using Escherichia coli (E. coli) and Salmonella enterica serovar Typhimurium (S. enterica serovar Typhimurium). Using intestinal porcine epithelial cells (IPEC-J2), the effects of pre-, co-, and post-treatment with Lactobacillus (L.) rhamnosus on the barrier function, intracellular (IC) reactive oxygen species (ROS) production, proinflammatory cytokine (IL-6 and IL-8) response, and adhesion inhibition were tested. E. coli- and S. Typhimurium-induced barrier impairment and increased ROS production could be counteracted using L. rhamnosus (p S. Typhimurium-induced IL-6 production was reduced via pre-treatment (p p p L. rhamnosus. L. rhamnosus demonstrated significant inhibition of adhesion for both S. Typhimurium (p E. coli (p p L. rhamnosus. Our findings can serve as a basis for further in vivo studies carried out in pigs and humans.

Keywords

• <i>Lactobacillus rhamnosus</i>
• <i>Escherichia coli</i>
• <i>Salmonella enterica</i> serovar Typhimurium
• IPEC-J2
• paracellular permeability
• ROS