Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries

Longwei Liu; Praopim Limsakul; Xianhui Meng; Yan Huang; Reed E. S. Harrison; Tse-Shun Huang; Yiwen Shi; Yiyan Yu; Krit Charupanit; Sheng Zhong; Shaoying Lu; Jin Zhang; Shu Chien; Jie Sun; Yingxiao Wang

doi:10.1038/s41467-021-25323-x

Nature Communications (Aug 2021)

Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries

Longwei Liu,
Praopim Limsakul,
Xianhui Meng,
Yan Huang,
Reed E. S. Harrison,
Tse-Shun Huang,
Yiwen Shi,
Yiyan Yu,
Krit Charupanit,
Sheng Zhong,
Shaoying Lu,
Jin Zhang,
Shu Chien,
Jie Sun,
Yingxiao Wang

Affiliations

Longwei Liu: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Praopim Limsakul: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Xianhui Meng: Department of Cell Biology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine
Yan Huang: Department of Chemistry and Chemical Engineering, Hunan University
Reed E. S. Harrison: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Tse-Shun Huang: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Yiwen Shi: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Yiyan Yu: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Krit Charupanit: Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University
Sheng Zhong: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Shaoying Lu: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Jin Zhang: Department of Pharmacology, University of California
Shu Chien: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Jie Sun: Department of Cell Biology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine
Yingxiao Wang: Department of Bioengineering, Institute of Engineering in Medicine, University of California

DOI: https://doi.org/10.1038/s41467-021-25323-x
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 16

Abstract

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Existing Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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