An in vitro method to survey DNA methylation in peripheral blood mononuclear cells (PBMCs) treated by airborne particulate matter (PM10)
Maryam Faraji,
Zahra Pourpak,
Kazem Naddafi,
Ramin Nabizadeh Nodehi,
Mohammad Hossein Nicknam,
Mansour Shamsipour,
Zahra Alizadeh,
Soheila Rezaei,
Narjes Soleimanifar,
Alireza Mesdaghinia
Affiliations
Maryam Faraji
Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Zahra Pourpak
Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Kazem Naddafi
Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran
Ramin Nabizadeh Nodehi
Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran
Mohammad Hossein Nicknam
Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Mansour Shamsipour
Department of Research Methodology and Data Analysis, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran
Zahra Alizadeh
Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Soheila Rezaei
Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Narjes Soleimanifar
Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Alireza Mesdaghinia
Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran; Corresponding author at: School of Public Health, Tehran University of Medical Sciences, Enqelab Square, Tehran, Iran.
The International Agency for Research on Cancer (IARC) has defined outdoor air pollution and PM as the human carcinogen (Group 1), which mainly cause an increased risk of lung cancer. Scientists have considered epigenetic modifications as a possible mechanism to deal with adverse effects of air pollution. This study aimed to compare the effect of airborne PM10 (PM with aerodynamic diameter ≤10 μm) on in vitro global methylation in human peripheral blood mononuclear cells (PBMCs). PM10 was sampled in metropolitan Tehran, the capital of Iran. PBMCs were extracted from whole blood of healthy males and treated with PM10 suspension at concentrations of 50–300 μg/mL for 4 h. Untreated cells were used as the negative control. Genomic DNA was extracted from each sample using the DNA blood mini kit according to the manufacturer’s instruction. Moreover, 5-methylsytosine (%5-mC) and 5-hydroxymethylcytosine (%5-hmC) were measured by the enzyme-linked immunosorbent assay (ELISA) method. %5-mC and %5-hmC in each sample was compared with negative control and reported as difference %5-mC and %5-hmC. Method name: Effect of airborne particulate matter on DNA methylation, Keywords: PM10 (PM with aerodynamic diameter ≤10 μm), Epigenetic, 5-Methylscytosine (5-mC), 5-Hydroxymethylscytosine (5-hmC), In vitro DNA methylation, Air pollution
