Noninvasive imaging of the lung NETosis by anti-Ly6G iron oxide nanoparticles
Jianghong Zhong,
Chanyu Zheng,
Haiqiang Gao,
Wei Tong,
Hui Hui,
Jie Tian
Affiliations
Jianghong Zhong
School of Engineering Medicine, Beihang University, Beijing 100191, China; Key Laboratory of Big Data-Based Precision Medicine (Beihang University), Ministry of Industry and Information Technology, Beijing, 100191, China; Corresponding author.
Chanyu Zheng
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China
Haiqiang Gao
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China
Wei Tong
CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China
Hui Hui
CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China
Jie Tian
School of Engineering Medicine, Beihang University, Beijing 100191, China; Key Laboratory of Big Data-Based Precision Medicine (Beihang University), Ministry of Industry and Information Technology, Beijing, 100191, China; CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; Corresponding author.
It is challenging to visualize noninvasively the formation of neutrophil extracellular traps, known as NETosis, and therefore difficult to monitor disease progression. A desirable molecular imaging probe is the iron oxide nanoparticle (NP) that could induce reactive oxygen species. Here, we used C57BL/6 mice with pristane-induced lupus, which mimics systemic lupus erythematosus. Administration of anti-Ly6G antibody-conjugated NP allowed detection of NETosis with fluorescent molecular imaging, as evidenced by flow cytometric analysis of citrullinated histone H3 expression in lung neutrophils. This finding was consistent with NP-induced blood NETosis in a spontaneous lupus model of B6.MRL-lpr mice. A chronic assessment was performed in which the lupus mice were protected from enhanced oxidative burst by anti-Ly6G NP. This NP can migrate from the peritoneal cavity to the lungs, as visualized by magnetic particle imaging. Overall, our study provides evidence for a highly sensitive assessment of NETosis in lupus through magnetic particle imaging.
