Cellular & Molecular Biology Letters (Sep 2017)

Luteolin, quercetin, genistein and quercetagetin inhibit the effects of lipopolysaccharide obtained from Porphyromonas gingivalis in H9c2 cardiomyoblasts

  • Gloria Gutiérrez-Venegas,
  • Alfredo Torras-Ceballos,
  • Juan Arturo Gómez-Mora,
  • Berenice Fernández-Rojas

DOI
https://doi.org/10.1186/s11658-017-0047-z
Journal volume & issue
Vol. 22, no. 1
pp. 1 – 12

Abstract

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Abstract Background One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is Porphyromonas gingivalis. It is a Gram-negative bacteria harvested from chronic periodontitis patients. Lipopolysaccharide (LPS) obtained from P. gingivalis promotes the expressions of interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α). Flavonoids are thought to participate in processes that control inflammation, such as the expression of cyclooxygenase-2 (COX-2). Methods We investigated the effects of luteolin, quercetin, genistein and quercetagetin on cardiomyoblasts treated with LPS alone or in combination with following inhibitors p38 (SB203580), ERK (PD98059), JNK (SP600125) and PKC (Calphostin C) for 1 h. The kinase activation and COX-2 expression levels were determined at the gene and protein levels. Results These flavonoids are considered to inhibit the activation of mitogen-activated protein kinase (MAPK) and the degradation of inhibitor of kappa B-alpha (IκB-α). They also play a role in COX-2 expression. Conclusion We conclude that the tested flavonoids inhibit inflammatory responses induced by LPS in H9c2 cells.

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