Data in support of qPCR primer design and verification in a Pink1 −/− rat model of Parkinson disease
Cynthia A. Kelm-Nelson,
Sharon A. Stevenson,
Michelle R. Ciucci
Affiliations
Cynthia A. Kelm-Nelson
Department of Surgery, Division of Otolaryngology, University of Wisconsin-Madison, Madison, WI, USA; Correspondence to: Department of Surgery, Division of Otolaryngology, 1300 University Avenue, 483 Medical Sciences Center, University of Wisconsin-Madison, Madison, WI 53706, USA. Tel.: +1 608 262 6122; fax: +1 608 262 6356.
Sharon A. Stevenson
Department of Zoology, University of Wisconsin-Madison, Madison, WI, USA
Michelle R. Ciucci
Department of Surgery, Division of Otolaryngology, University of Wisconsin-Madison, Madison, WI, USA; Neuroscience Training Program, University of Wisconsin-Madison, Madison, WI, USA; Department of Communication Sciences and Disorders, University of Wisconsin-Madison, Madison, WI, USA
Datasets provided in this article represent the Rattus norvegicus primer design and verification used in Pink1 −/− and wildtype Long Evans brain tissue. Accessible tables include relevant information, accession numbers, sequences, temperatures and product length, describing primer design specific to the transcript amplification use. Additionally, results of Sanger sequencing of qPCR reaction products (FASTA aligned sequences) are presented for genes of interest. Results and further interpretation and discussion can be found in the original research article “Atp13a2 expression in the periaqueductal gray is decreased in the Pink1 −/− rat model of Parkinson disease” [1].
