Comparative study of Moringa stenopetala root and leaf extracts against the bacteria Staphyloccocus aureus strain from aquatic environment.

C.S. Metsopkeng; M.E. Nougang; P.A. Nana; A. Tamsa Arfao; P. Ngo Bahebeck; C. Lontsi Djimeli; J.S. Eheth; O.V. Noah Ewoti; L.M. Moungang; G.A. Agbor; F. Perrière; T. Sime-Ngando; M. Nola

Scientific African (Nov 2020)

Comparative study of Moringa stenopetala root and leaf extracts against the bacteria Staphyloccocus aureus strain from aquatic environment.

C.S. Metsopkeng,
M.E. Nougang,
P.A. Nana,
A. Tamsa Arfao,
P. Ngo Bahebeck,
C. Lontsi Djimeli,
J.S. Eheth,
O.V. Noah Ewoti,
L.M. Moungang,
G.A. Agbor,
F. Perrière,
T. Sime-Ngando,
M. Nola

Affiliations

C.S. Metsopkeng: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon
M.E. Nougang: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon; Zoology laboratory, Faculty of Sciences, University of Maroua, P.O.Box 814 Maroua, Cameroon
P.A. Nana: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon; Department of Oceanography and Limnology, Institute of Fisheries and Aquatic Sciences, University of Douala, P.O.Box 7236, Douala, Cameroon
A. Tamsa Arfao: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon
P. Ngo Bahebeck: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon; Laboratory of Soils, Plants, Fertilizer and Water analysis, IRAD, P.O. Box 2123 Yaounde, Cameroon
C. Lontsi Djimeli: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon
J.S. Eheth: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon
O.V. Noah Ewoti: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon
L.M. Moungang: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon
G.A. Agbor: Center for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon
F. Perrière: Laboratoire ‘Microorganismes: Génome et Environnement’, UMR CNRS 6023, Université Clermont Auvergne, Campus Universitaire des Cézeaux, 1 Impasse Amélie Murat CS 60026, 63178 Aubière Cedex, France
T. Sime-Ngando: Laboratoire ‘Microorganismes: Génome et Environnement’, UMR CNRS 6023, Université Clermont Auvergne, Campus Universitaire des Cézeaux, 1 Impasse Amélie Murat CS 60026, 63178 Aubière Cedex, France
M. Nola: Laboratory of Hydrobiology and Environment, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon; Corresponding author.

Journal volume & issue: Vol. 10
p. e00549

Abstract

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The present study assessed the abiotic factors of Moringa stenopetala (M. stenopetala) extracts against Staphyloccoccus aureus in aquatic microcosm. Bacterial survival was assessed using colony forming unit (CFU) method on culture agar medium at different concentration levels (0.5 to 2 g/L). The incubation duration varied from 3 to 24 hours, and the temperatures were between 7°C and 44°C. Phytochemical screening showed that most of the constituents obtained from the extracts are the alkaloids, flavonoids and the tannins. It was observed that increase in extract concentration significantly inhibited the number of CFU obtained. Compared to the control {9.27 Log(CFU/100 mL)}, cells abundance from different concentrations of leaves extracts fluctuated between 4.74 and 0 {Log(CFU/100 mL)} after 3h incubation; 5.46 and 0 {Log(CFU/100 mL)} after 6h; 4.75 and 0 {Log(CFU̸/100 mL)} after 9h, and 4.85 and 0 {Log(CFU/100 mL)} after 24h incubation. As for root extracts, the abundance were between 4.80 and 0 {Log(CFU/100 mL)} under 7°C incubation; 5.03-5.23 and 0 {Log(CFU/100 mL)} under 23°C and 37°C, and 4.66 and 0 Log (CFU/100 mL) under 44°C incubation when considering the whole extract concentrations used. Incubation temperature and extracts concentration significantly impacted bacterial abundances. When the decoction extracts of roots were used, the amount of S. aureus cells inhibited was registered after 3-24 hours incubation. The Pearson correlation coefficient in most cases, revealed an inversely proportional relationship between the plant extracts and S. aureus cultured cells in a dose-dependent manner (P≤0.05). The use of M. stenopetala extracts could constitute an alternative in the treatment of water intended for human consumption.

Published in Scientific African

ISSN: 2468-2276 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science
Website: https://www.journals.elsevier.com/scientific-african

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