Nature Communications (Nov 2022)
PPARγ lipodystrophy mutants reveal intermolecular interactions required for enhancer activation
- Maria Stahl Madsen,
- Marjoleine F. Broekema,
- Martin Rønn Madsen,
- Arjen Koppen,
- Anouska Borgman,
- Cathrin Gräwe,
- Elisabeth G. K. Thomsen,
- Denise Westland,
- Mariette E. G. Kranendonk,
- Marian Groot Koerkamp,
- Nicole Hamers,
- Alexandre M. J. J. Bonvin,
- José M. Ramos Pittol,
- Kedar Nath Natarajan,
- Sander Kersten,
- Frank C. P. Holstege,
- Houshang Monajemi,
- Saskia W. C. van Mil,
- Michiel Vermeulen,
- Birthe B. Kragelund,
- David Cassiman,
- Susanne Mandrup,
- Eric Kalkhoven
Affiliations
- Maria Stahl Madsen
- Functional Genomics and Metabolism Research Unit, Department of Biochemistry and Molecular Biology, University of Southern Denmark
- Marjoleine F. Broekema
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Martin Rønn Madsen
- Functional Genomics and Metabolism Research Unit, Department of Biochemistry and Molecular Biology, University of Southern Denmark
- Arjen Koppen
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Anouska Borgman
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Cathrin Gräwe
- Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen
- Elisabeth G. K. Thomsen
- Department of Biology, University of Copenhagen
- Denise Westland
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Mariette E. G. Kranendonk
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Marian Groot Koerkamp
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Nicole Hamers
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Alexandre M. J. J. Bonvin
- Faculty of Science—Chemistry, Bijvoet Centre for Biomolecular Research, Utrecht University
- José M. Ramos Pittol
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Kedar Nath Natarajan
- Functional Genomics and Metabolism Research Unit, Department of Biochemistry and Molecular Biology, University of Southern Denmark
- Sander Kersten
- Division of Human Nutrition, Nutrition, Metabolism and Genomics Group, Wageningen University
- Frank C. P. Holstege
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Houshang Monajemi
- Internal Medicine, Rijnstate Hospital
- Saskia W. C. van Mil
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- Michiel Vermeulen
- Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen
- Birthe B. Kragelund
- Department of Biology, University of Copenhagen
- David Cassiman
- Center for Metabolic Diseases, Leuven University Hospitals
- Susanne Mandrup
- Functional Genomics and Metabolism Research Unit, Department of Biochemistry and Molecular Biology, University of Southern Denmark
- Eric Kalkhoven
- Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University
- DOI
- https://doi.org/10.1038/s41467-022-34766-9
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 19
Abstract
Mutations in PPARγ lead to lipodystrophy, but the mechanisms by which the mutations affect the activity in chromatin is unknown. Here, Madsen, Broekema et al. showed that mutations affecting two intermolecular interactions compromise chromatin remodeling.
