Generation of induced pluripotent stem cells from three individuals with Huntington‘s disease

Duncan C. Miller; Pawel Lisowski; Selene Lickfett; Barbara Mlody; Miriam Bünning; Carolin Genehr; Claas Ulrich; Erich E. Wanker; Sebastian Diecke; Josef Priller; Alessandro Prigione

Stem Cell Research (Dec 2022)

Generation of induced pluripotent stem cells from three individuals with Huntington‘s disease

Duncan C. Miller,
Pawel Lisowski,
Selene Lickfett,
Barbara Mlody,
Miriam Bünning,
Carolin Genehr,
Claas Ulrich,
Erich E. Wanker,
Sebastian Diecke,
Josef Priller,
Alessandro Prigione

Affiliations

Duncan C. Miller: Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany
Pawel Lisowski: Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Magdalenka n/Warsaw, Poland; Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité – Universitätsmedizin Berlin, Germany
Selene Lickfett: Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany
Barbara Mlody: Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany
Miriam Bünning: Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany
Carolin Genehr: Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany
Claas Ulrich: Department of Dermatology and Allergy, Charité – Universitätsmedizin Berlin, Germany
Erich E. Wanker: Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany
Sebastian Diecke: Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany; Corresponding authors.
Josef Priller: Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité – Universitätsmedizin Berlin, Germany; DZNE, Berlin, Germany; University of Edinburgh and UK DRI, Edinburgh, UK; Department of Psychiatry and Psychotherapy, School of Medicine, Technical University Munich, Germany; Corresponding authors.
Alessandro Prigione: Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany; Corresponding authors.

Journal volume & issue: Vol. 65
p. 102976

Abstract

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Huntington’s disease (HD) is a neurodegenerative disorder caused by abnormal glutamine (Q) expansion in the huntingtin protein due to elongated CAG repeats in the gene HTT. We used non-integrative episomal plasmids to generate induced pluripotent stem cells (iPSCs) from three individuals affected by HD: CH1 (58Q), and two twin brothers CH3 (44Q) and CH4 (44Q). The iPSC lines exhibited one healthy HTT allele and one with elongated CAG repeats, as confirmed by PCR and sequencing. All iPSC lines expressed pluripotency markers, exhibited a normal karyotype, and generated cells of the three germ layers in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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