Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain

Abstract

When a monkeypox virus outbreak began in several parts of the world in May 2022, timely and accurate diagnosis became mandatory. In our laboratory, a real-time quantitative PCR was designed and evaluated in several patient samples and compared with isolation results. Genomic viral load was related to virus viability.

Keywords

• mpox
• monkeypox virus
• orthopoxvirus
• real-time PCR
• cell culture
• sexually transmitted infections