Cumhuriyet Dental Journal (Mar 2022)
THE EFFECTS OF DIFFERENT DESENSITIZERS AND THEIR COMBINATIONS WITH ER, CR: YSGG LASER ON DENTIN TUBULES, AND SHEAR BOND STRENGTH TO DENTIN
Abstract
AIM: The aim of this in vitro study was to investigate the effectiveness of Sodium Fluoride, Propolis and Er,Cr:YSGG laser alone and in combination with laser applications on dentin tubules by SEM and to examine the effects of these applications on the shear bond strength (SBS) of a universal adhesive to dentin. METHODS: Dentin samples obtained by removing the buccal enamel of 72 caries-free permanent, mandibular third molars were randomly divided into six groups (n:12): Control, Sodium Fluoride (Enamelast, Ultradent), Propolis (Fanus Propolis), Er,Cr:YSGG Laser (Biolase, Waterlase), Enamelast-Er,Cr:YSGG Laser, Propolis-Er,Cr:YSGG Laser. While the agents were applied to the dentin surfaces in the agents alone groups, laser was applied after the agent applications in the combination groups. No application was performed to the control group. After storing the samples in artificial saliva for 14 days, Clearfil Tri-S Bond Universal (Kuraray) and composite resin (Estelite Sigma Quick, Tokuyama) were applied to their surfaces. SBS tests were conducted using the Universal Tensile-Compression Test System (Instron 3382, USA). The tubule plugging efficiencies of the agents were examined on two dentin samples from each group by SEM. One-way analysis of variance (One-way ANOVA) was performed for statistical analysis of the data. For p<0.05, the results were considered statistically significant. RESULTS: No statistically significant difference was found in any comparison between all the test groups (p>0.05). In SEM examinations, occluded dentinal tubules were observed more frequently in the groups where the applications were performed in combination with laser, compared to the application of the agents alone. In the propolis group too, the tubules were usually closed, as well as open dentin tubules. CONCLUSIONS: Although combination applications of Er,Cr:YSGG Laser with Sodium Fluoride and Propolis showed greater dentin tubule plugging efficiency, these applications did not have a negative effect on the SBS of Clearfil Tri-S Bond Universal compared to the control group. The changes caused by the laser application on the dentin surface and the content of adhesive system may have prevented the SBS from being adversely affected.
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