ISOLATION OF NEWCASTLE DISEASE VIRUS (NDV) IN EMBRYONATED CHICKEN EGGS

Abstract

Newcastle disease virus (NDV) strains have been divided into three groups: virulent(velogenic), moderately virulent (mesogenic), and non-virulent strains (lentogenic). The nonvirulentvirus strain (LaSota strain) has been used as a live vaccine, which gives good immunityagainst the virulent strains. The aim of the study was to grow and propagate Newcastle diseasevirus in the lab, determination ofcytopathic effects in chicken embryos, and confirmation ofvirus growth by hemagglutination test. Non-virulent strain (LaSota strain)represented by livevaccine was used for this purpose. Embryonated eggs were inoculated with the virus andincubated for 48 hours; and theallantoic fluids were then collected for further processing.Petichial hemorrhages were clearly observed in the embryos following infection while in theun-inoculated eggs; the embryos appeared normal and did not show any lesions. For furthervirus growth confirmation, the presence of virus in the allantoic fluid was determined byhemagglutiation test. This finding is considered as a starting point for Newastle disease virusantigen preparation, which is essential for the applications of several laboratory techniques.

Keywords

• newcastle disease virus
• vaccine
• hemagglutination