Viruses (Apr 2024)

Establishment of the First National Standard for Neutralizing Antibodies against SARS-CoV-2 XBB Variants

  • Xuanxuan Zhang,
  • Lidong Guan,
  • Na Li,
  • Ying Wang,
  • Lu Li,
  • Mingchen Liu,
  • Qian He,
  • Jiansheng Lu,
  • Haiyuan Zeng,
  • Shan Yu,
  • Xinyi Guo,
  • Jiali Gong,
  • Jing Li,
  • Fan Gao,
  • Xing Wu,
  • Si Chen,
  • Qian Wang,
  • Zhongfang Wang,
  • Weijin Huang,
  • Qunying Mao,
  • Zhenglun Liang,
  • Miao Xu

DOI
https://doi.org/10.3390/v16040554
Journal volume & issue
Vol. 16, no. 4
p. 554

Abstract

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Neutralizing antibodies (NtAbs) against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are indicators of vaccine efficacy that enable immunity surveillance. However, the rapid mutation of SARS-CoV-2 variants prevents the timely establishment of standards required for effective XBB vaccine evaluation. Therefore, we prepared four candidate standards (No. 11, No. 44, No. 22, and No. 33) using plasma, purified immunoglobulin, and a broad-spectrum neutralizing monoclonal antibody. Collaborative calibration was conducted across nine Chinese laboratories using neutralization methods against 11 strains containing the XBB and BA.2.86 sublineages. This study demonstrated the reduced neutralization potency of the first International Standard antibodies to SARS-CoV-2 variants of concern against XBB variants. No. 44 displayed broad-spectrum neutralizing activity against XBB sublineages, effectively reduced interlaboratory variability for nearly all XBB variants, and effectively minimized the geometric mean titer (GMT) difference between the live and pseudotyped virus. No. 22 showed a broader spectrum and higher neutralizing activity against all strains but failed to reduce interlaboratory variability. Thus, No. 44 was approved as a National Standard for NtAbs against XBB variants, providing a unified NtAb measurement standard for XBB variants for the first time. Moreover, No. 22 was approved as a national reference reagent for NtAbs against SARS-CoV-2, offering a broad-spectrum activity reference for current and potentially emerging variants.

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