Évaluation de la biodiversité de quelques cucurbitacées d’Afrique subsaharienne à partir des données chromatographiques (CGC et CLHP)

Abstract

Two triacylglycerol quantitative analysis methods, needing none standard, using liquid chromatography coupled with light scattering detection, as well as fatty acid methyl esters quantitative analysis using capillary gas chromatography were led. In application, seed oil saponifiable fraction composition of eight groups of cucurbitacee’s seeds from equatorial and west Africa was determinated Their correct classification and their differentiation using respectively factorial discriminent and principal component analysis were investigated. It is demonstrated that the best method for characterizing an oil is the new original triacylglycerol quantitative analysis reported in this article. The interest of the two other methods is also reported.

Keywords

• triacylglycerols
• quantitative analysis without standard
• HPLC
• ELSD
• PCA
• FDA
• cucurbitacees