The F(ab′)2 fragment of an Aβ-specific monoclonal antibody reduces Aβ deposits in the brain

Abstract

This work examines whether administering the F(ab´)2 fragment of an IgG1 monoclonal antibody (mAb) targeting the N-terminal 1–13 amino acids of the β-amyloid peptide (Aβ mAb) reduces amyloid deposition in Alzheimer's disease (AD). The F(ab′)2 fragment was injected intraperitoneally or intracranially into Tg2576 mice, a murine model of human AD. Both routes of administration significantly reduced Aβ plaque formation in the brain, as determined immunohistochemically and by monitoring levels of Aβ1–40 and Aβ1–42 peptide. Use of the F(ab′)2 fragment significantly reduced phagocytic infiltration in the CNS when compared to intact mAb. Since IgG1 Abs do not fix complement, these findings suggest that effective in vivo clearance of amyloid deposits can be achieved without stimulation of FcR-reactive phagocytes or activation of the complement cascade.

Keywords

• Alzheimer's disease
• Tg2576 mouse
• β-amyloid peptide (Aβ)
• F(ab′)2 fragment
• IgG1 isotype
• Anti-Aβ monoclonal antibody