Frontiers in Molecular Biosciences (Feb 2022)

Down-Regulated miR-130a/b Attenuates Rhabdomyosarcoma Proliferation via PPARG

  • Yi Pan,
  • Junyang Li,
  • Junyang Li,
  • Susu Lou,
  • Wanbiao Chen,
  • Wanbiao Chen,
  • Yihang Lin,
  • Yihang Lin,
  • Nan Shen,
  • Nan Shen,
  • Youjin Li

DOI
https://doi.org/10.3389/fmolb.2021.766887
Journal volume & issue
Vol. 8

Abstract

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Background: Rhabdomyosarcoma (RMS) is one of the most common types of soft-tissue sarcomas in children, and it exhibits a low 5-years survival rate. The survival outcome has shown no significant improvements in the past 30 years miRNA profiling of RMS might therefore provide a novel insight into uncovering new molecular targets for therapy.Methods: We analyzed miRNA and RNA sequencing data from patients and the TARGET database to reveal the potential miRNA-mRNA axes and validated them in patients’ samples. After the miRNA antagomirs were used to silence the target miRNAs in the cell model, qRT-PCR, western immunoblotting analysis, and proliferation assays were performed to explore the interaction between miR-130a/b and peroxisome proliferator-activated receptor gamma (PPARG) and their effects.Results: In RMS patients, the expression of miR-130a/b was augmented, and its related PPARG gene was suppressed. Bioinformatics analysis showed that miR-130a/b targeted the PPARG gene and inhibited the proliferation of human RMS cell lines. In addition, rosiglitazone maleate activated the expression of PPARG in human RMS cell lines to suppress proliferation.Conclusion: miR-130a/b regulates the malignant process in RMS by targeting PPARG. Furthermore, the PPARG agonist rosiglitazone maleate attenuated the proliferation of RD cells and might therefore be of benefit to RMS patients.

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