Sensors (Apr 2008)

Visible Genotype Sensor Array

  • Takashi Imai,
  • Mayumi Iwakawa,
  • Kenichi Ishikawa,
  • Atsuko Ishikawa,
  • Izumi Matsumoto,
  • Yoshimi Ohtsuka,
  • Tomo Suga,
  • Yuichi Michikawa

Journal volume & issue
Vol. 8, no. 4
pp. 2722 – 2735

Abstract

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A visible sensor array system for simultaneous multiple SNP genotyping has been developed using a new plastic base with specific surface chemistry. Discrimination of SNP alleles is carried out by an allele-specific extension reaction using immobilized oligonucleotide primers. The 3’-ends of oligonucleotide primers are modified with a locked nucleic acid to enhance their efficiency in allelic discrimination. Biotin-dUTPs included in the reaction mixture are selectively incorporated into extending primer sequences and are utilized as tags for alkaline phosphatase-mediated precipitation of colored chemical substrates onto the surface of the plastic base. The visible precipitates allow immediate inspection of typing results by the naked eye and easy recording by a digital camera equipped on a commercial mobile phone. Up to four individuals can be analyzed on a single sensor array and multiple sensor arrays can be handled in a single operation. All of the reactions can be performed within one hour using conventional laboratory instruments. This visible genotype sensor array is suitable for “focused genomics” that follows “comprehensive genomics”.

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