Chitosan/Hyaluronate Complex-Coated Electrospun Poly(3-hydroxybutyrate) Materials Containing Extracts from Melissa officinalis and/or Hypericum perforatum with Various Biological Activities: Antioxidant, Antibacterial and In Vitro Anticancer Effects

Ina Anastasova; Milena Ignatova; Nevena Manolova; Iliya Rashkov; Nadya Markova; Reneta Toshkova; Ani Georgieva; Mariana Kamenova-Nacheva; Antoaneta Trendafilova; Viktoria Ivanova; Tsvetelina Doncheva

doi:10.3390/polym16152105

Polymers (Jul 2024)

Chitosan/Hyaluronate Complex-Coated Electrospun Poly(3-hydroxybutyrate) Materials Containing Extracts from Melissa officinalis and/or Hypericum perforatum with Various Biological Activities: Antioxidant, Antibacterial and In Vitro Anticancer Effects

Ina Anastasova,
Milena Ignatova,
Nevena Manolova,
Iliya Rashkov,
Nadya Markova,
Reneta Toshkova,
Ani Georgieva,
Mariana Kamenova-Nacheva,
Antoaneta Trendafilova,
Viktoria Ivanova,
Tsvetelina Doncheva

Affiliations

Ina Anastasova: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 103A, BG-1113 Sofia, Bulgaria
Milena Ignatova: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 103A, BG-1113 Sofia, Bulgaria
Nevena Manolova: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 103A, BG-1113 Sofia, Bulgaria
Iliya Rashkov: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 103A, BG-1113 Sofia, Bulgaria
Nadya Markova: Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 26, BG-1113 Sofia, Bulgaria
Reneta Toshkova: Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 25, BG-1113 Sofia, Bulgaria
Ani Georgieva: Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 25, BG-1113 Sofia, Bulgaria
Mariana Kamenova-Nacheva: Laboratory for Extraction of Natural Products and Synthesis of Bioactive Compounds, Research and Development and Innovation Consortium, Sofia Tech Park JSC, 111 Tsarigradsko Shose blvd., BG-1784 Sofia, Bulgaria
Antoaneta Trendafilova: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 9, BG-1113 Sofia, Bulgaria
Viktoria Ivanova: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 9, BG-1113 Sofia, Bulgaria
Tsvetelina Doncheva: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev St., Bl. 9, BG-1113 Sofia, Bulgaria

DOI: https://doi.org/10.3390/polym16152105
Journal volume & issue: Vol. 16, no. 15
p. 2105

Abstract

Read online

The present study aimed to fabricate innovative fibrous materials with various biological activities from poly(3-hydroxybutyrate), sodium hyaluronate (HA), chitosan (Ch), Melissa officinalis (MO), Hypericum perforatum (HP) extract, or a combination of both extracts. Electrospinning or electrospinning followed by dip coating and the subsequent formation of a polyelectrolyte complex were the methods used to prepare these materials. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and attenuated total reflection–Fourier transform infrared spectroscopy (ATR–FTIR) were applied for investigating the morphology of materials, their thermal characteristics, and their surface chemical composition. The composition and design of the mats had an influence on the in vitro release behavior of the main bioactive compounds present in the MO and HP extracts incorporated in the materials. It was found that as-created materials comprising a combination of both extracts and a Ch/HA complex exerted higher antioxidant activity than that of (non-)coated MO-containing mats and Ch/HA-coated mats containing HP. The novel materials manifested antibacterial efficacy towards the pathogenic bacteria S. aureus and E. coli, as evidenced by the performed microbiological screening. Furthermore, the mats possessed a great growth inhibitory effect on HeLa cancer cells but had a less pronounced effect on the growth of normal mouse BALB/3T3 fibroblasts. The loading of both extracts in the mats and the formation of coating led to the enhancement of the in vitro anticancer and antibacterial activities of the materials. Thus, the novel materials have potential for use in local cancer therapy as well as for use as wound dressings.

Published in Polymers

ISSN: 2073-4360 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/polymers/

About the journal

Abstract

Keywords